In this study we investigate the composition of Late Roman glass and sand bodies in the vicinity of a newly discovered primary glass production site, unearthed during an excavation near Tel Yavne, Israel. The site offers a unique opportunity to examine natron glass production in the southern Israeli coastline during the Late Roman period. Additionally, it allows the exploration of potential raw material sources due to its proximity to various sandy deposits fed by the Nile-coastal sedimentary system of the southern Levant. A comprehensive investigation of sand bodies and Roman glass using geochemical, mineralogical, and grain size methodologies, reveals primary glass pieces and sand samples collected along a transect from the modern coast to the production site at Yavne. This study demonstrates that any of the examined sand bodies could have provided the required quartz sand for glass production in Yavne. A comparison of the Yavne glass findings with known glass groups from the Levant and Egypt reveals some similarities and differences in glass production compared to other known sites of glass production at that time. A novel aspect of this study is the direct comparison of glass compositions with various adjacent sand deposits, which are often missing when Roman glass is investigated outside of the Levant, alongside a review of sand mineralogy in existing literature. This study highlights the unique features of the Levantine Yavne glass group, which are similar to other sites (e.g., Jalame) along the Israeli coastline. Moreover, our chemical investigation points to the possible addition of another ingredient, that enriched the Yavne glass with calcium and aluminum, and was never considered before. Such novel suggestions demonstrate the importance of comparing potential raw materials with their products using multi-disciplinary approaches.